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Real-time fluorescence quantitative PCR (RT qPCR) is the mostadvanced clinical diagnostic techniques for analysis of nucleic and molecules. It is generally recognized by the U.S. FDA and many respected platforms have acquired the rights for clinical application.

 

PCR products were labeled with specific probes that have fluorescent dyes or are fluorescently labeled. The reaction process is monitored in real-time using online software. The reaction products are then analyzed to calculate the initial concentration of the sample template. Per amplification of a DNA strand, there will be a fluorescent molecule formation. Finally, the full synchronization will be achieved from the accumulation of the fluorescent signal and the PCR product formation.

 

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